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Fluorescence recovery after photobleaching (FRAP) of mCherry-CAS in the nucleus of a HEK293T cell. The measurement was conducted 24 h after transfection using a point scanning confocal “LSM880 Inverted” microscope with Airyscan (Zeiss). Frame rate, 0.2 fps (one frame per 5 s). In total, 120 frames were recorded within 600 s. These data were used in the nuclear FRAP analysis shown in Fig. 8 C.
