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. 2023 Nov 29;43(4):235–247. doi: 10.1038/s41388-023-02901-5

Fig. 8. Functional consequences of ECM remodeling for stromal and epithelial cells.

Fig. 8

A Quantification of the adhesion capacity and representative microscopy images of PF179T-CAF cells after short-term culture with 100 nM Dex for 3 d and 6 d and pre-treatment with trypsin for 2 min before measurement. Data represent mean + SEM from 3 independent experiments (one-way ANOVA and correction for multiple testing using Bonferroni’s comparison test; *P < 0.05; ***P < 0.001). B Quantification of the adhesion capacity and representative microscopy images of PF179T-CAF cells after long-term culture with 100 nM Dex for 15 d and 30 d and pre-treatment with trypsin for 2 min before measurement. Data represent mean + SEM from 3 independent experiments (one-way ANOVA and correction for multiple testing using Bonferroni’s comparison test; *P < 0.05; **, P < 0.01). C Quantification of the re-attachment capacity and representative microscopy images of PF179T-CAF cells in a period of 60 min after treatment with 100 nM Dex or 100 nM Pred for 3 d and 6 d. Data represent mean + SEM from 3 independent experiments (one-way ANOVA and correction for multiple testing using Bonferroni’s comparison test; ***P < 0.001). D Quantification of the re-attachment capacity and representative microscopy images of CWR22Rv1 cells in a period of 60 min on a confluent PF179T-CAF cell monolayer. PF179T-CAF cells were pre-treated with 100 nM Dex or 100 nM Pred for 6 d before measurement. Data represent mean cell numbers + SEM from 3 independent experiments (one-way ANOVA and correction for multiple testing using Bonferroni’s comparison test; **P < 0.01; **P < 0.01; Box Whisker Plot with 10-90 percentile). E Schematic illustration of the proposed impact of altered soluble factor expression and ECM remodeling under the influence of GCs on tumor cell growth and disease progression based on the presented findings. The illustration was created in PowerPoint with the help of the MOTIFOLIO illustration tool kit.