Table 3.

NTRK IHC and results of RNA-sequencing

Scoring group	Totally negative	Focal, weak	Focal, moderate/strong	Widespread, weak	Widespread, moderate/strong
NTRK TMA N (%)	878 (93.4%)	19 (2.0%)	17 (1.8%)	17 (1.8%)	9 (1.0%)
Mean/median H-score (range)	0	24.2/20 (10–40)	58.5/50 (15–120)	94.7/90 (50–150)	223.3/220 (140–300)
RNA NGS number analyzed	62	1	17	17	9
--Fusions	ALK: 1 RET: 1 ROS1: 3 MET ex. 14 skip: 1 Negative: 54 Failed: 2	Negative: 1	FGFR3: 1 Negative: 15 Failed: 1	Negative: 16 Failed: 1	MYB: 1 Negative: 8

No NTRK fusions were found with NGS. The two positive salivary gland carcinomas had strong and widespread positive IHC, and one had a MYB-NFIB fusion and the other a ERBB2 mutation

Met ex 14 skip = Met exon 14 skipping

CNV, copy number variation