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Cross-reactivity for dual aptassay of PCT (A) and IL-6 (B). For PCT: a) 0 PCT; b) 0 PCT + 2 µg/mL IL-6; c) 3 µg/mL PCT; d) 3 µg/mL PCT + 2 µg/mL IL-6. For IL-6: a) 0 IL-6; b) 0 IL-6 + 3 µg/mL PCT; c) 2 µg/mL IL-6; d) 2 µg/mL IL-6 + 3 µg/mL PCT. Fluorescence intensity was recorded at the selective wavelengths of aptamer-tagged labels (Alexa405 Apt PCT, λem = 447 nm and Alexa488 6-FAM) (Apt IL-6, λem=520 nm), n = 3. Other conditions: (see Table 1)
