Table 3.

Flask cultivation with host strain and single knockout engineered strain

Strains	Lactate (g/L)	Formate (g/L)	Acetate (g/L)	Succinate (g/L)	Pyruvate (g/L)	Ethanol (g/L)	Acetoin (g/L)	2,3-BDO (g/L)	Glucose (g/L)
IAM1183-L	0.11 ± 0.01	0.54 ± 0.03	0.62 ± 0.01	0.52 ± 0.03	0.10 ± 0.01	0.52 ± 0.02	2.51 ± 0.08	10.52 ± 0.50	3.65 ± 0.18
IAM1183-P	1.39 ± 0.03	0.57 ± 0.05	0.88 ± 0.02	0.35 ± 0.07	0.19 ± 0.01	0.50 ± 0.03	1.84 ± 0.10	12.06 ± 0.46	2.14 ± 0.28
IAM1183-C	2.80 ± 0.26	1.41 ± 0.02	0.62 ± 0.05	0.21 ± 0.02	0.22 ± 0.01	0.44 ± 0.01	1.47 ± 0.10	10.90 ± 0.84	7.25 ± 0.83
IAM1183-T	2.07 ± 0.22	1.13 ± 0.04	0.34 ± 0.01	1.33 ± 0.12	0.19 ± 0.01	1.53 ± 0.13	2.07 ± 0.09	11.91 ± 0.40	1.88 ± 0.13
IAM1183-H	1.73 ± 0.13	1.43 ± 0.13	1.44 ± 0.05	1.16 ± 0.09	0.18 ± 0.01	0.38 ± 0.01	1.14 ± 0.08	7.72 ± 0.14	5.69 ± 0.44
IAM1183 (wild type)	1.87 ± 0.03	1.52 ± 0.04	1.38 ± 0.02	0.42 ± 0.06	0.18 ± 0.01	1.19 ± 0.11	2.80 ± 0.17	7.02 ± 0.51	3.67 ± 0.13