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. 2024 Jan 19;14:1729. doi: 10.1038/s41598-024-51365-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CIII does not mask CI operation during anoxia. (A) Scheme illustrating the action of inhibitor(s) used downstream of CI in the experiments shown in the figure (created with BioRender.com). Quantification of inhibitor-induced changes in UQ (B,C) or NADH (D,E) signal. Vehicle or inhibitors present as indicated by the plus ( +) signs at the bottom of the panels. Substrates were glutamate and malate (5 mM each) present in the buffer prior to addition of mitochondria. Eth ethanol (grey), Myx myxothiazol, 0.1 μM (orange), Rot rotenone, 1 μM (blue). The additions of the compounds mentioned in the bottom of each panel were made from top to bottom. Data pooled from 3 to 37 independent experiments. *p < 0.05.