Figure 6.

Acute anoxia and targeted inhibition of the ETS exert no impact on the stability of complex I or on the formation of supercomplexes. Western blot and immunodetection of Blue-Native electrophoresis gels. The formation of respiratory supercomplexes containing CI was determined in liver mitochondria from 129/Sv and from C57Bl/6J mice in normoxia and in either anoxia alone or combined with treatment with different inhibitors (Rot rotenone; Atpn atpenin A5; Myx myxothiazol; KCN potassium cyanide). The main bands detected correspond to free complex I (CI) and to the supercomplex formed by the association of complexes I and dimeric III (SC I+III₂). The higher molecular bands of lower intensity correspond to the association of complexes I, dimeric III and IV, also known as the ‘respirasomes’ (SC I+ I+III₂+IV). Complex II (CII), migrating as a single entity of approximately 140 kDa, was also immunodetected in the same samples, to serve as a loading control.