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. 2024 Jan 19;14:1703. doi: 10.1038/s41598-024-51978-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Immunity Clock applied to mice after positive lifestyle interventions. (B) Redox Clock applied to mice after positive lifestyle interventions. (C) Kaplan Meier cumulative survival of O Control, O AKK and O SE. (D) Kaplan Meier cumulative survival of PAM C and PAM SE. (E) ImmunolAge vs lifespan in old mice after nutritional and social interventions. Pearson’s correlation coefficient between ImmunolAge and Longevity is − 0.713 (p < 0.001). (F) RedoxAge vs lifespan in old mice after nutritional and social interventions. Pearson’s correlation coefficient between RedoxAge and lifespan is − 0.570 (p < 0.01). (G) ImmunolAge vs lifespan in adult PAM after social interventions. Pearson’s correlation coefficient between ImmunolAge and lifespan is − 0.828 (p < 0.001). (H) RedoxAge vs lifespan in PAM after social interventions. Pearson’s correlation coefficient between RedoxAge and Longevity is − 0.812 (p < 0.001). O Control, Old control; O AKK, old mice after Akkermansia ingestion; O SE, old mice after cohabitation with adult mice; A Control, Adult control; PAM C, prematurely aging mice control; PAM SE, prematurely aging mice after cohabitation with exceptional non-prematurely aging mice. *p < 0.05, **p < 0.01, ***p < 0.001 respect O Control. + p < 0.05 respect A Control. ##p < 0.01, ###p < 0.001 respect PAM C. a p < 0.05, aa p < 0.01 respect NPAM.