FIG. 7.

SDS-PAGE analysis of rHtrA and analogs during purification performed on 12.5% polyacrylamide gels. Lanes 1, prestained molecular weight markers; lanes 2, total cellular proteins from E. coli BL21(DE3) expressing rHtrA or mutant proteins; lanes 3, crude extract of recombinant protein; lanes 4, flowthrough fraction from DEAE Sephacel column for soluble rHtrA and H91A; lanes 5, purified protein after the HTP column. The numbers at the left of each row represent molecular weights in thousands.