TABLE 2.
Antibody isotypes elicited by immunization with rHtrA and its analogs
| Isotype | Antibody titera for protein:
|
||
|---|---|---|---|
| rHtrA | H91A | S197A | |
| IgG (H+L) | 10 | 10 | 10 |
| IgG1 | 8 | 8 | 8 |
| IgG2a | <1 | <1 | <1 |
| IgG2b | 1.5 | <1 | 2 |
| IgG3 | <1 | <1 | <1 |
a
IgG isotype titers of sera collected on day 54 after three immunizations were determined by ELISA. The reactive titer was defined as the reciprocal of the dilution consistently showing a twofold increase in absorbance over that obtained with the prebleed serum sample and is expressed as log2(titer/100). H + L, heavy plus light chains.