Fig. 2.
The expression of PTX3 is suppressed in colon cancer cells due to a regulation of epigenetic silencing. A, B The mRNA expression and secreted protein levels of PTX3 in CCD-18Co human colon fibroblasts, and 7 human colon cancer cell lines. C, D The mRNA expression and secreted protein levels of PTX3 in mouse embryonic fibroblasts (MEFs) and the mouse colon cancer cell line MC38. The conditioned medium was harvested for ELISA assay (n = 3). E–H PTX3 mRNA and protein in CCD-18Co cells, MEFs, SW620 cells, and MC38 cells were measured by real-time PCR and western blot, respectively. The experimental cells were treated with IL-1β or TNF-α for 48 h. I–L PTX3 mRNA and protein in HCT116, HT29, SW620, and MC38 cells were measured by real-time PCR and western blot, respectively. The experimental cells were treated with 5-azacytidine for 4 days and stimulated with 20 ng/ml IL-1β or TNF-α for 24 h beginning on the 3rd day. GAPDH as internal control. p values were calculated by two-tailed unpaired Student’s t test or one-way ANOVA. ns represents nonsignificant difference. *represents a p value of < 0.05. **represents a p value of < 0.01. ***represents a p value of < 0.001. Values on the plots are presented as the means ± SEMs. Data are combined from 3 to 5 independent experiments