Fig. 5.

CircNUP50 upregulates G3BP1 by acting as a miR-197-3p sponge. A Heatmap of miRNA sequencing of platinum-resistant and PS OC tissues. B KEGG analysis based on miRNA-seq data. C Identification of miRNAs that bind to circNUP50. D miR-197-3p expression is significantly different in platinum-resistant and PS OC tissues. E miR-197-3p expression is significantly different in platinum-resistant and PS cells. F circRNA pull-down assay showed that miR-197-3p could specifically bind to circNUP50. G RIP experiments showed increased circNUP50 and miR-197-3p enrichment in the Ago2 group compared to that in the IgG group. H RNA FISH experiments showed that circNUP50 and miR-197-3p were co-localised in the cytoplasm. I Identification of specific target genes of miR-197-3p from three databases. J After combining si-circNUP50 mRNA-seq data, two target genes of miR-197-3p were identified. K–M Luciferase activity was significantly inhibited in cells co-transfected with miR-197-3p mimic and wild-type luciferase reporter genes (circNUP50-WT and G3BP1-WT) compared to that in the controls