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IL-12 secretion from cultured splenocytes collected at 6 d (A) ; 9 d (B) post SPPV immunization. Splenic macrophages were harvested from mice (five/group), cultured with LPS 1 μg/ml for 48 h. IL-12 was measured in the culture supernatant. Bars represent mean ± S:E:M: of cytokine. SPPV vaccinated mice are significantly different from controls at *P < 0.05 or **P < 0.01.
