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. Author manuscript; available in PMC: 2024 Mar 20.
Published in final edited form as: Nature. 2023 Sep 20;621(7980):804–812. doi: 10.1038/s41586-023-06526-2

Fig. 2 |. Identification of a sutural DDR2+ CSC.

Fig. 2 |

a, DDR2+ cells, LAM, Ctsk-Cre;mTmG mice, P7. b, FACS, P7. Pregated on LinThy-1.26C3. c, DDR2, WT and Twist1Ctsk;mTmG mice, P14 (i), iDTRCtsk mice, P21 (ii). d, DDR2+ CSCs by FACS (Lin6C3Thy-1.2CTSK-mGFPCD200+CD105CD51+DDR2+) in the sutures of WT (n=13), Twist1Ctsk (n=20), P7 to P10 (i), and iDTRCtsk mice, PBS (n=4) or DT (n=3) at P21 (ii). e, In vivo intramuscular serial transplantation. f, FACS of DDR2+ CSC-derived cells after serial transplantation. Black arrows, parent/daughter gates. Red boxes, DDR2+ CSCs. Representative of 4 independent experiments. g, FACS showing no interconversion between tdTomato+ DDR2+ CSCs and mGFP+ CTSK+ CSCs isolated from Ctsk-Cre;mTmG mice after the first round of transplantation. h, Lineage tracing of sutural DDR2+ cells using Ddr2-CreER;mTmG mice over an 18 month chase. i, FACS plots showing total H2B-GFP label-retaining cells after 6 months of chase post-doxycycline (Dox). j, GFP signal intensity histogram of LinThy-1.26C3DDR2+ or DDR2 populations after 6 (n=9) or 12 months (n=8) of chase. Label-retaining cells (LRCs) were determined as gated. Green, CD200+CD105 (CSC); Blue, CD200variableCD105+; Red, CD200CD105. k, LAM, H2B-GFP mice after 6 months of chase with DDR2 immunostaining. In all graphs, each of dot represents an individual mouse. ****P < 0.0001. Mean ± s.d., unpaired two-tailed Student’s t-test (d). Images in a, c, h, k are representative of at least three independent experiments. Scale bars, 100 μm, a, c, h (left), k; 50 μm, a (far-right); 20 μm, c, h (right), k (inset).