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. 2005 Apr 14;33(7):2181–2191. doi: 10.1093/nar/gki513

Figure 3.

Figure 3

Analysis of NER activities of UvrABC and E.coli CFEs for Oxa and Oxa–Sp. (A) 60G, 60OXAe, 60OXA–SPe and 60FL (all 2 nM and 5′-end labeled, Figure 1B) were incubated with UvrABC (20 nM UvrA, 60 nM UvrB and 50 nM UvrC) at 37°C for 1 h. Products were analyzed by 12% denaturing PAGE. The sequence of the 23mer marker is given in Figure 1B. (B) 60OXAi and 60OXA–SPi (all 2 nM and internally labeled, Figure 1B) were incubated with UvrABC, and products were analyzed as in panel A. The sequences of the 12mer and 25mer markers are given in Figure 1B. (C) 60OXA–SPe and 60 FL were incubated with UvrABC as described in panel A for up to 30 min. The amounts of 5′ incision products are plotted against incubation time. Symbols: circles, Oxa–Sp; triangles, FL. (D) 60OXA–SPe (2 nM) was incubated at 37°C for 15 min with CFEs (2, 5 and 10 μg) from E.coli BW9109 (xth) and RPC501 (xth nfo) that were pre-irradiated without (−SOS) or with (+SOS) UV. Products were analyzed as in panel A.