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. 2024 Jan 20;10:6. doi: 10.1038/s41522-024-00479-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Fluorescent changes of biosensor strains under varying environmental signals were measured using microplate reader or predicted in silico modelling. b–d Biosensor strains were modelled (up) under regulation of pLldR (lactate response), pCadC (pH response) and pPepT (hypoxic response) promoters and compared with (down) in vitro experimental results (n = 3, ± s.e.m). The fluorescent results of mRFP were normalized by OD₆₀₀. e–g In presence of lysis gene, in vitro mRFP fluorescence of biosensor strains were compared with mathematic modeling prediction (n = 3, ± s.e.m). Detailed equations and parameters used in this study could be obtained in supplementary materials.