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. 2024 Jan 20;15:622. doi: 10.1038/s41467-024-44872-5

Fig. 4. GST-LBD fusion protein or antisera against GST-LBD inhibit GETV infection in different cell lines.

Fig. 4

a, b Virions of rGETV-EGFP were pre-incubated with increasing concentrations of GST-LBD or GST and then used to infect BHK-21 cells. At 18 h post infection number of infected cells was measured by flow cytometry (a) and virus titers in supernatant were determined using titration on BHK-21 cells (b). For panel a values obtained for cells infected with mock-treated virions were taken as 100%. Data are presented as mean values ± SD (n = 3 independent experiments) and two-tailed P-values are calculated by unpaired Student’s t test. c, d Virions of rGETV-EGFP were pre-incubated with GST-LBD or GST proteins taken at concentration 10 μg ml-1 or mock-treated and used to infect ST, LLC-PK1 or Vero cells. At 18 h post infection number of infected cells was measured by flow cytometry (c) and virus titer were determined using titration on BHK-21 cells (d). For panel c values obtained for cells infected with mock-treated virions were taken as 100%. Data are presented as mean values ± SD (n = 3 independent experiments) and two-tailed P-values are calculated by unpaired Student’s t test. e–h Cultures of BHK-21 (e and f), ST, LLC-PK1 or Vero cells (g and h) were preincubated with serial dilutions of anti-LDLR polyclonal mouse serum or an isotype control (naïve mice serum) before infection with rGETV-EGFP at an MOI of 0.001 for BHK-21 or 0.1 for ST, LLC-PK1 and Vero cells. At 18 h post infection the number of infected cells was measured by flow cytometry (e and g), and viral titer were determined using titration on BHK-21 cells (f and h). For panels e and g values obtained for control cells were taken as 100%. Data are presented as mean values ± SD (n = 3 independent experiments) and two-tailed P-values are calculated by unpaired Student’s t test. Source data are provided as a Source Data file.