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. 2024 Jan 21;14:1822. doi: 10.1038/s41598-024-52319-6

Table 2.

Methods used for different soil biochemical analysis.

Soil biochemical properties Method used References
pH pH meter Datta et al. (1997)
EC electronic EC meter Datta et al. (1997)
OC Wet oxidation method Walkley and Black (1934)
Available N Kjeldahl method Subbiah and Asija (1956)
Available P Olsens method Olsen et al. (1954)
Available K Flame photometer method Hanway and Heidel (1952)
Soil microbial count (107 cfu/g soil) Standard serial dilution technique on soil extract media Wright (1933)
Diazotrophic count (104 cfu/g soil) Standard serial dilution technique on nutrient agar media Wright (1933)
Actinomycetes count (105 cfu/g soil) Standard serial dilution technique on Kenknights media HiMedia (2009)
Dehydrogenase activity Triphenylformazon through reduction of 2,3,5 triphenyltetrazolium chloride Casida et al. (1964)
Alkaline phosphatase activity n-nitrophenyl method Tabatabai and Bremner (1969)