FIG. 1.

Colocalization of intracellular growth mutants with late endosomal, lysosomal marker LAMP-1 in mouse bone marrow-derived macrophages by immunofluorescence microscopy. Macrophages were infected with wild-type or mutant strains of L. pneumophila for 2 h, fixed, and stained for LAMP-1 colocalization (A, C, E, G, and I) and intracellular versus extracellular bacteria (B, D, F, H, and J). Neighboring panels show LAMP-1 staining (left) and corresponding intracellular bacteria (right). (A and B) Lp01 (dot⁺); (C and D) formalin-killed Lp01 (dot⁺); (E and F) HL1400 (dotO); (G and H) HL1700 (dotH); (I and J) HL056 (dotI).