FIG. 2.

Intracellular growth mutants of L. pneumophila colocalize with LAMP-1. For each sample, mouse bone marrow macrophages were incubated with mutant or wild-type strains for 2 h, fixed, stained for intracellular versus extracellular bacteria and LAMP-1 colocalization, and examined. Data were collected from 100 intracellular bacteria in total. Percent LAMP-1 positive was calculated by dividing the number of intracellular rod-shaped bacteria colocalizing with LAMP-1 by the total number of intracellular rod-shaped bacteria scored. Values shown are averages of duplicate samples from two identical experiments (four samples in total) and their standard deviations.