TABLE 1.
Strains and plasmids used
| Strain or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| L. pneumophilaa | ||
| Lp01 | Philadelphia-1 rpsL hsdR | 2 |
| Lp02 | Lp01 thyA | 2 |
| HL019 | Lp01/pMS8 | This work |
| HL900 | Lp01 dotG? | This work |
| HL1000 | Lp01 dotO2 | This work |
| HL1005 | Lp01 dotO2/pMS8 | This work |
| HL1009 | Lp01 dotO2/p1415 | This work |
| HL1011 | Lp01 dotO2/p1A | This work |
| HL1012 | Lp01 dotO2/p2A | This work |
| HL1300 | Lp01 Dot− | This work |
| HL1400 | Lp01 dotO1 | This work |
| HL1405 | HL1400/pMS8 | This work |
| HL1419 | HL1400/p1415 | This work |
| HL1421 | Lp01 dotO1/p1A | This work |
| HL1422 | Lp01 dotO1/p2A | This work |
| HL1600 | Lp01 Dot− | This work |
| HL1700 | Lp01 dotH1 | This work |
| HL1705 | HL1700/pMS8 | This work |
| HL1719 | HL1700/p1713 | This work |
| HL1725 | HL1700/pH3 | This work |
| HL056 | Lp01 dotIΔ1 | This work |
| HL057 | HL056/pMS8 | This work |
| HL059 | HL056/pI1 | This work |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 49 |
| DH5α (λpir) | DH5α (λpir) tet::Mu recA | 24 |
| XL1-Blue | F′::Tn10 proA+B+ lacIq Δ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17 (rK− mK+) supE44 relA1 lac | 5 |
| MT607 | recA56 pro-82 thi-1 hsdR17 supE44, RK600 | 14 |
| Plasmids | ||
| pMS4 | oriRSF1010 RP4 mob kan | 39 |
| pMS8 | pMS4 sacB | 37 |
| p1415 | pMS8 proP+ dotO+ | This work |
| p1A | pMS8 proP′ | This work |
| p2A | pMS8 dotO+ | This work |
| p1713 | pMS8 dotH+ dotI+ | This work |
| pH3 | pMS8 dotH+ | This work |
| pI1 | pMS8 dotI+ | This work |
| pSR47 | oriTRP4 oriR6K kan | 27 |
| pSR47S | pSR47 sacB | 45a |
| pHJK | pSR47s dotH+ dotJ+ dotK+ | This work |
a
All were derived from Philadelphia-1, serogroup 1 strain Lp01 (CDC, Atlanta, Ga.).