Fig. 3.

Characterization of single terminator candidates. Terminator candidates (Table 1) were cloned into a vector carrying the firefly luciferase coding sequence and the 35S promoter. Terminators were characterized via Dual-Luciferase assay and obtained firefly-luc/Renilla-luc reporter values of single candidates normalized to the 35S terminator. Blue dots represent means of three technical replicates of a biological replicate. For each terminator n ≥ 3 biological replicates were analysed. Bars represent means of all biological replicates with standard deviations. Significance levels are based on a one-way ANOVA (p = 7.91E−11, α = 0.05) with subsequent Tukey HSD post hoc test. Indicated differences are between 35St and the respective terminator (*p < 0.05; **p < 0.005; ***p < 0.0005)