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. 2024 Jan 22;43(2):43. doi: 10.1007/s00299-023-03088-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Characterization of double terminators. Single terminators were fused in homologous and heterologous pairs in vectors bearing the firefly luciferase coding sequence and the 35S promoter. Double terminators and their respective single terminators were characterized via Dual-Luciferase assays and obtained firefly-luc/Renilla-luc reporter values normalized to the 35S terminator. Blue dots represent means of three technical replicates of a biological replicate. Bars represent means of all biological replicates (n ≥ 3) with standard deviations. No significant difference in performance were observed for combinations of the 35St and NOSt (A one-way ANOVA, p = 0.11, α = 0.05). Significant differences in performance were observed in combinations of PpCab2t and PppetEt (B one-way ANOVA, p = 4.16E−7, α = 0.05) and in combinations of AtHSP90t and PpRPS6et (C one-way ANOVA, p = 8.42E−05, α = 0.05). Significance levels and compact letter display are based on a one-way ANOVA with subsequent Tukey HSD post hoc test