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. 2024 Jan 22;43(2):43. doi: 10.1007/s00299-023-03088-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Characterization of terminators in combination with the NOS or the PpActin5 promoter. A selection of single terminators was cloned in vectors bearing the firefly luciferase coding sequence and either the A NOS promoter or the B PpActin5 promoter. The promoter–terminator combinations were characterized via Dual-Luciferase assays and obtained firefly-luc/Renilla-luc reporter values normalized to a testing vector bearing the 35S promoter and 35S terminator. Blue dots represent means of three technical replicates of a biological replicate. Bars represent means of all biological replicates (n ≥ 3) with standard deviations. In combination with the NOS promoter, only terminator PpnoIUK2t shows significant difference in comparison to 35St (A, one-way ANOVA, p = 0.0012, α = 0.05). For the PpActin5 promoter, no tested terminator significantly differed in performance from 35St (B, one-way ANOVA, p = 0.046, α = 0.05). Significance levels are based on a one-way ANOVA with subsequent Tukey HSD post hoc test. Indicated differences are between 35St and the respective terminator (*p < 0.05)