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. 2024 Jan 8;11:1290693. doi: 10.3389/fbioe.2023.1290693

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Copyright © 2024 Taguchi, Lopez and Takawira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construct cell ultrastructure. Electron microphotographs of cells from ASC-COLI constructs cultured in stromal (A) or tenogenic (B) medium for 21 days with fibrils in the ECM surrounding the latter (yellow arrows). Cells within constructs cultured in stromal medium had abundant rough endoplasmic reticulum (white arrow) and mitochondria (black arrow), and heterochromatin was apparent in the nuclei of construct cells cultured in tenogenic medium (gray arrow). Nuclei are indicated with an asterisk (*) and template material with a hash (#). Scale bars = 2 μm (A), 1 μm (B).