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. 2024 Jan 8;11:1290693. doi: 10.3389/fbioe.2023.1290693

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Copyright © 2024 Taguchi, Lopez and Takawira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Perfusion bioreactor system. Medium perfusion was driven by a computer-controlled peristaltic pump connected to the bioreactor chamber (1) and a medium reservoir (2). The pump was attached with 1.0 mm tubing (5a) to a 0.22 µm syringe filter which was connected to a segment of 4.8 mm tubing (4a) attached to one port on the upper surface of a 10 mL medium reservoir (2). Another port on the upper surface of the medium reservoir was attached to the lower port of the bioreactor chamber with 4.8 mm tubing (6). The pump was also connected with 1.0 mm tubing (5b) to another 0.22 µm syringe filter (small black arrow) which was connected with a 3-way stopcock (large black arrow) 4.8 mm tubing (4b) attached to the upper port on the bioreactor chamber (1). A magnetic stir bar at the lowest end of the bioreactor chamber driven by a stir plate (3) provided centrifugal fluid motion.