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. 2024 Jan 8;11:1290693. doi: 10.3389/fbioe.2023.1290693

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Copyright © 2024 Taguchi, Lopez and Takawira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tendon-related gene expression of construct cells. Fold change (mean ± SEM) in mRNA levels of tenogenic transcription factors Scx, Mkx, Egr1, CTGF, and LOX (A), tenogenic ECM genes Col1a1, Col3a1, Dcn, Eln, Tnc, and Bgn (B), and mature tendon markers, Fbmd, Col14a1, and THBS4 (C) by cells in COLI-ASC constructs cultured in tenogenic medium relative to those cultured in stromal medium for 7 (purple), 14 (yellow), or 21 (green) days. Asterisks indicate a difference from 1-fold within each culture period.