FIGURE 9.

Immunohistochemical fibromodulin staining. Fibromodulin (Fbmd, green) localization in COLI-ASC constructs cultured in stromal (left) or tenogenic (right) medium for 7, 14, or 21 days, and in a section of deep digital flexor tendon (DDFT). A construct section stained with only secondary antibody (2° Ab only) is also shown. Scant, non-specific staining (gray arrow) was apparent after 14 days in constructs cultured in tenogenic medium, and, after 21 days of culture in tenogenic medium, rings of fibromodulin surrounded well-circumscribed regions within constructs (white arrow). Cell clusters (blue) were apparent in all constructs. DAPI counterstain; Scale bar = 100 μm.