TABLE 6.
Mean IC50 and FIC50 of AD01+artemisone and PhX6+artemisone against CQ-sensitive Plasmodium falciparum NF54 strain using the fixed ratio isobologram method.
| Combination | Combination ratio (A:B) | a Mean IC50 ± SD (nM) | a Mean FIC50 | ∑FIC50 | Interaction | ||
|---|---|---|---|---|---|---|---|
| Drug A | Drug B | Drug A | Drug B | ||||
| AMS + AD01 | 1:0 | 6.16 ± 2.92 | nd | 1.00 ± 0.0 | nd | 1.00 | |
| 3:1 | 3.40 ± 1.44 | 0.92 ± 0.40 | 0.56 ± 0.08 | 0.04 ± 0.02 | 0.60 | Synergy | |
| 1:1 | 1.97 ± 0.83 | 2.04 ± 0.78 | 0.34 ± 0.07 | 0.09 ± 0.04 | 0.43 | Synergy | |
| 1:3 | 1.14 ± 0.38 | 3.63 ± 1.53 | 0.20 ± 0.05 | 0.16 ± 0.08 | 0.36 | Synergy | |
| 1:9 | 1.35 ± 0.54 | 10.35 ± 2.40 | 0.25 ± 0.13 | 0.46 ± 0.15 | 0.71 | Synergy | |
| 0:1 | nd | 23.31 ± 2.49 | nd | 1.00 ± 0.0 | 1.00 | ||
| AMS + PhX6 | 1:0 | 2.86 ± 1.01 | nd | 1.00 ± 0.0 | nd | 1.00 | |
| 3:1 | 1.84 ± 0.59 | 3.20 ± 1.34 | 0.65 ± 0.02 | 0.09 ± 0.05 | 0.73 | Synergy | |
| 1:1 | 1.46 ± 0.46 | 7.88 ± 3.67 | 0.51 ± 0.06 | 0.20 ± 0.09 | 0.72 | Synergy | |
| 1:3 | 0.91 ± 0.31 | 14.17 ± 6.13 | 0.32 ± 0.01 | 0.38 ± 0.20 | 0.70 | Synergy | |
| 1:9 | 0.61 ± 0.35 | 21.21 ± 2.22 | 0.21 ± 0.05 | 0.67 ± 0.17 | 0.87 | Addition | |
| 0:1 | nd | 45.61 ± 5.69 | nd | 1.00 ± 0.0 | 1.00 | ||
a
Results shown are the mean IC50 values and SD, from at least three independent experiments.