Figure 3.

Assessment of off-target editing

On-target or off-target editing at top in silico-nominated candidate sites calculated as the proportion of aligned sequencing reads with ≥1 adenine base edited to guanine within the editing window at each site in PAH c.1222C>T homozygous HuH-7 cells that underwent treatment with SpRY-ABE8.8/PAH4 LNPs at a dose of 10,000 fg/cell (n = 3 treated and 3 untreated biological replicates), the highest dose shown in Figure 1C. Sites with unsuccessful sequencing are omitted. Refer to Table S2 for candidate site sequences and numerical values.