Figure 3.

Deletion of TFEB has minor effects on energy homeostasis in LVH. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of indicated metabolic genes (A–B) and genes involved in OXPHOS (C) and mitochondrial fission and fusion (D) from WT and cKO mice after 21 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh. (E) Representative images of PAS stained heart cross-sections of WT and cKO mice after 21 days of Sham or TAC surgery; left: overview, scale bare 1 mm; middle: higher magnification (corresponding to inset from left), scale bar 100 µm, right: quantification of glycogen content with Image J. Data are presented as mean ± SD (21 days, WT Sham: n = 10, WT TAC: n = 13, cKO Sham: n = 10, cKO TAC: n = 15; *q < 0.05, **q < 0.01, ***q < 0.001, ****q < 0.0001).