Figure 5.

Deletion of TFEB reduces cardiac remodeling in pressure overload-induced HFrEF. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of Nppa and Nppb (A) and Myh6, Myh7, and Acta1 (B) expression from WT and cKO mice after 56 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh. (C) Western blot analysis with anti-β-MyHC and anti-GAPDH antibodies. GAPDH was used as loading control. Bar graph showing the ratio of the relative densities of β-MyHC and GAPDH protein contents. (D) Representative images of Picrosirius Red stained (PSR, left) heart cross-sections of WT and cKO mice after 56 days of Sham or TAC surgery; scale bar, 1 mm. Fibrotic area (right) was measured with Image J. (E) qRT-PCR analysis of indicated genes from WT and cKO mice after 56 days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh. Data are presented as mean ± SD (56 days, WT Sham: n = 10, WT TAC: n = 6, cKO Sham: n = 8, cKO TAC: n = 6; ^*,”,&q < 0.05 **q < 0.01, ***q < 0.001, ****q < 0.0001).