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. 2024 Jan 8;10:1323760. doi: 10.3389/fcvm.2023.1323760

Figure 6.

Figure 6

Deletion of TFEB has minor effects on metabolic remodeling and energy homeostasis in HFrEF. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of indicated metabolic genes (A–B) and genes involved in OXPHOS (C) and mitochondrial fission and fusion (F) from WT and cKO mice after 56days of Sham or TAC surgery as indicated. mRNA expression was normalized to Gapdh. (D) Citrate synthase and (E) complex II activity in protein lysates from hearts of WT and cKO mice after 56 days of Sham or TAC surgery. (G) Representative images of PAS stained heart cross-sections of WT and cKO mice after 56 days of Sham or TAC surgery; left: overview, scale bare 1 mm; middle: higher magnification (corresponding to inset from left), scale bar 100 µm, right: quantification of glycogen content with Image J. Data are presented as mean ± SD (56 days, WT Sham: n = 10, WT TAC: n = 6, cKO Sham: n = 8, cKO TAC: n = 6; *q < 0.05, **q < 0.01, ***q < 0.001, ****q < 0.0001).