Figure 3.

A biomimetic microfluidic assay that reproduces complete microvascular networks on a chip to study the effect of inflammation and sepsis on endothelial permeability and of neutrophil-endothelial cell interaction (A); the dark spot in the middle of (A) is the port for connecting tubing to the tissue compartment. The vascular channels of this system are cultured with primary human endothelial cells (B) and the neutrophil-endothelial cell interaction can be studied under physiologically relevant shear stress levels in the presence of chemoattractants such as fMLP in the tissue compartment. [(B) reproduced with permission (Soroush et al., 2020)].