Figure 2.

Locomotion parameters in an open field test over the lifespan of the African turquoise killifish. 30-min recordings were carried out and fish were observed in an open field test every three weeks from age 6 weeks until age 24 weeks. (A) Total distance moved in a 30-min recording shown for the different ages. (B) Mean velocity and (C) Mean mobility (movement of the fish even if the center point remains in the same location). (D,E) Total distance moved and (F,G) mean mobility as a function of fish age and time (1-min time bins) to investigate habituation over time. 12- and 15-week-old fish decreased their distance moved (E) and mean mobility (G) over the 30-min recording, and hence show habituation (yellow and green lines). Points represent individual fish in panels (A,B). Points represent the means of the different age groups in panels (C,D). n = 14–16 per age group. w = weeks. Significant differences are indicated with an asterisk.