Figure 3.

Exploration-related parameters in an open field test over the lifespan of the African turquoise killifish. 30-min recordings were carried out and fish were observed in an open field test every three weeks from the age of 6 weeks until the age of 24 weeks. (A) The total time spent in the center zone during a 30-min recording for the different ages shows that upon aging, fish spent more time in the center. (B) The total duration of immobility, and (C) the distance moved in the center divided by the total distance moved as a function of age. With age, fish moved a greater distance in the center zone. Time spent in the center (D), immobility (E), and the percentage distance moved in the center (F) as a function of fish age and time (1-min time bins). (E,G) Habituation was only observed for the 12- and 15-week-old fish, where immobility increased over time (yellow and green lines). Immobility is defined as a velocity lower than 0.1 cm/s for a duration of at least 1 s. (F) The distance moved in the center divided by the total distance moved, shows the percentage of distance moved in the center. Points represent individual fish in panels (A–C). Points indicate means of the different fish per age group in panels (D–G). n = 14–16 per age group. w = weeks. Significant differences are indicated with an asterisk. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.