Figure 4.

Locomotion and exploration-related parameters in the novel tank diving test do not reveal any clear aging effect. Total distance moved (A), mean velocity (B), and mean mobility (C) as a function of age. (D) Time spent in the bottom half (E) upper half, and (F) bottom 25% in function of the different fish ages. (G) Immobility frequency and duration (H) as a function of fish age. (I) Latency to reach the upper half as a function of fish age. Immobility is defined as a velocity lower than 0.1 cm/s for a duration of at least 1 s. (J,K) Representative traces and heatmaps of different fish ages over the 30 min recording. Pink indicates the upper half and yellow the bottom half. High presence is indicated in red in the heatmap. n = 10–14 per age group. w = weeks. Significant differences are indicated with an asterisk. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.