Figure 2.

Groupwise comparison between HBEC cell lines and EGFR mutant NSCLC cell lines. (A) Schematic showing comparison performed during statistical analysis and volcano plot of log₂fold change (LFC) differentially expressed proteins identified from MS/MS analysis. The top 20 significantly over- and under-expressed proteins (p adj< 0.05 and absolute LFC > 0.6) are colored in red or blue, respectively, and labeled. (B) Heatmap of differentially expressed proteins identified from group-wise comparison in A., with hierarchical clustering (n=91) (cell line and protein, hierarchical clustering; cell line clustering distance, complete; protein clustering distance, average). (C) Bar plots showing the top 20 GO enrichment terms sorted by adjusted p-value (p adj< 0.05, Benjamini-Hochberg adjustment) for biological processes, molecular functions, and cellular components for differentially expressed proteins found during MS/MS analysis.