Table 1.

Comparison of consensus amino acid sequences of H19 and avian, bat, and mammalian H9 HAs (H9 numbering).

Host	Receptor-binding sites										Bat influenza H9-specific sites
	166	191	197	198	232	234	235	236	399		5	14	15	17	18	28	32	35
Bat	N	H	E	Q	G	Q/R	G	R	K		K	Q	I	K/R	G	N	D	N
Avian H19	N	N	E	Q	G	Q	G	R	K		A	S	P	K	A	T	D	N
Avian H9	N/G	H	E/V/A	Q	G	Q/I	G	R	K/R		S/A/T	T/G	V/A/T	N	A	T	E	D
Swine	N	H	V	Q	G	Q	G	R	K		S	T	V	N	A	T	E	D
Human	N	H	V	Q	G	Q	G	R	K		P	T	A	N	A	T	E	D
Host	Bat influenza H9-specific sites
	38	40	46	47	52	53	54	58	61	62	63	64	66	69	71	72	74	75
Bat	I	S	S	S	E	S	S	L	S	N	E	K	S	V	T	S/N	L	V
Avian H19	I	N	Q	S	H	Q	E	L	S	T	S	K	S	E	D	K	K	I
Avian H9	T/V/I	N/T	H	A/T	H	E	E	M	A	T	N/D	L/Q	R/H	I	D	T	T	I/V
Swine	T	N	H/Q	A	H	E	E	M	A	T	N	L	H	I	D	T	T	I
Human	T	N	Q	A	H	E	E	M	A	T	N	L	H	I	D	T	T	I
Host	Bat influenza H9-specific sites
	84	87	90	102	104	107	112	113	126	127	133	137	139	166	173	177	181	182
Bat	Q	S	D/N	N	T	V	K	I	I	E	V	Q	V	T	E	R	G/K	H
Avian H19	E	L	G	T	Q	I	N	I	S	S	V	P	V	S	E	K	S	D
Avian H9	S	L	G	S	V/I	M/L	N/R	V	A/S	S/R	Q/L	P	I/T	N/S	D	T	G	K
Swine	S	L	G	S	V	M/L	N	V	A	S	Q	P	I	N	D	T	G	K
Human	S	L	G	S	V	M	N	V	A	S	Q	P	I	N	D	T	G	K
Host	Bat influenza H9-specific sites
	197	200	208	220	224	243	244	252	255	269	273	280	281	282	283	289	294	300
Bat	S	N/S	S	S	A	G	I	K	T	K	Y	E	T	P	E	E	F	S
Avian H19	S	E	T	S	T	G	I	K	T	R	H	A	AQ	P	L	E	K	S
Avian H9	T	T/M	T	T/V	V	S	V	R	S	S	H	D	L	K/N/S	S	Q	K/R	T
Swine	T	T	T	T	L/V	S	V	R	S	S	H	D	L	N	S	Q	R	T
Human	T	T	T	T	V	S	V	R	S	S	H	D	L	N	S	Q	R	T
Host	Bat influenza H9-specific sites
	313	319	320	324	327	335	336	337	367	368	376	383	395	401	405	413	415	418
Bat	Y	K	T	R	T	I	Q	T	A	E	Q	L	N	F	G	Q	I	S/L
Avian H19	N	R	T	K	L	K	E	T	S	E	L	I	D	Y	G	T	I	M
Avian H9	N/N	R/G	V	K	V	K/T/R/A	S	D/S/G	D	Q	R/K	V/I	D	Y	D	A/T	L	M
Swine	N	G	V	K	V	R	S/L	S	D	Q	R	I	D	Y	D	T	L	M
Human	N	G	V	K	V	R	S	S	D	Q	R	I	D	Y	D	T	L	M
Host	Bat influenza H9-specific sites
	426	429	438	454	458	463	466	469	472	477	479	483	485	496	498	501	518	519
Bat	V	L	I	R/K	D	S	E	K	D	Q	L	N	E	I/M	S	E	E	N
Avian H19	I	I	V	R	E	S	E	I	G	E	L	N	S	S	Y	S	S	N
Avian H9	I	I	V	N	N	A	S	I/M/R/V	G	E	Y	D	Q	D/N	Q/R	Q/K	S	E
Swine	I	I	V	N	N	A	S		G	E	Y	D	Q	N	R	K	S	E
Human	I	I	V	N	N	A	S		G	E	Y	D	R	N	G	K	S	E
Host	Bat influenza H9-specific sites						NGlyc sites										Cleavage site
	525	538	545	546	557		29	105	141	192	218	248	298	305	492		333
Bat	M	T	F	I	T		NSTD	–	NVTY	–	–	–	NTSL	NISK	NGTY		PAIQTR
Avian H19	L	L	M	V	T		NSTD	–	NVTY	NPSS	–	NQTL	NASL	NISK	NGTY		PIKETR
Avian H9	L	A	M/L	F	I/N		NSTE	NG(T/M/L)C	NV(T/S)Y	–	NRTF	–	NTTL	N(V/I)SK	NGTY		PA(R/A)SDR
Swine	L	A	L	F	N		NSTE	–	NVSY	–	NRTF	–	NTTL	NVSK	NGTY		PAR(S/L)SR
Human	L	A	L	F	N		NSTE	–	NVTY	–	NR(A/T)F	–	NTTL	NVSK	NGTY		PARSSR