TABLE 5.

Sensors discussed in Section 5.2 for detection of intracellular IP3 and DAG, and PKC activity.

GPCR signalling step	Detection method/mechanism	References
IP3 generation	CFP/YFP FRET	Tanimura et al. (2004), Tanimura et al. (2009)
	EGFP/tetramethylrhodamine FRET	Matsu-Ura et al. (2019)
DAG generation	CFP/YFP FRET “DAGR”	Violin et al. (2003)
	CFP/YFP FRET “Daglas”	Sato et al. (2006)
	RLuc/GFP BRET	Namkung et al. (2018)
PKC activity	CFP/YFP FRET “CKAR”	Violin et al. (2003)
	RLuc/GFP BRET	Namkung et al. (2018)