FIG. 1.

(A) Proteins from whole-cell extracts (2 μl of a 100-μl sample) and culture supernatants (3 μl of a 500-μl sample) from independent broth cultures (13, 24, 36, and 46 h) of H. pylori 85P were separated by SDS–12.5% PAGE and stained with Coomassie brilliant blue. Lane T, sterile BHI. (B) Protein analysis of water-washed cells (5 μl of a 600-μl sample) and water extracts (5 μl of a 200-μl sample) prepared after 24 and 48 h of culture as described in Materials and Methods is shown. The positions and sizes (in kilodaltons) of the protein standards and the positions of UreA, UreB, and HspB proteins and the multimeric forms of the HspA protein are indicated.