Table 3.
Changes in the leaf content of non-structural carbohydrates in Coffea canephora cv.
| Cultivar | Water | Temperature (day/night) | ||||
|---|---|---|---|---|---|---|
| 25/20°C | 31/25°C | 37/28°C | 42/30°C | Rec14 | ||
| Raffinose (mg g-1 DW) | ||||||
| CL 153 | WW | 0.64 ± 0.26 aB | 1.74 ± 0.37 aAB | 0.62 ± 0.11 aB | 0.78 ± 0.33 aB | 2.19 ± 0.56 aA |
| SWD | 0.62 ± 0.23 aA | 0.68 ± 0.11 aA | 0.11 ± 0.05 aA | 0.41 ± 0.13 aA | 0.95 ± 0.13 aB | |
| Icatu | WW | 1.1 ± 0.19 aB | 0.78 ± 0.3 aB | 2.72 ± 0.67 aAB | 4.63 ± 1.24 aA | 2.03 ± 0.26 aB |
| SWD | 1.06 ± 0.34 aA | 1.37 ± 0.54 aA | 1.1 ± 0.3 aA | 0.28 ± 0.18 bA | 1.57 ± 0.1 aA | |
| Trehalose (mg g-1 DW) | ||||||
| CL 153 | WW | 3.59 ± 0.94 aA | 0.07 ± 0.04 bC | 0.94 ± 0.3 aBC | 0.33 ± 0.17 aBC | 1.84 ± 0.31 aB |
| SWD | 1.49 ± 0.38 bA | 1.3 ± 0.17 aA | 0.95 ± 0.12 aA | 0.64 ± 0.2 aA | 0.49 ± 0.12 bA | |
| Icatu | WW | 0.39 ± 0.11 aA | 0.49 ± 0.28 aA | 0.38 ± 0.24 aA | 1.13 ± 0.45 aA | 0.7 ± 0.4 aA |
| SWD | 0.58 ± 0.18 aA | 0.12 ± 0.08 aA | 0.84 ± 0.41 aA | 0.7 ± 0.19 aA | 0.49 ± 0.14 aA | |
| Sucrose (mg g-1 DW) | ||||||
| CL 153 | WW | 20.7 ± 1.2 aA | 25.9 ± 1.6 aA | 18.1 ± 3.9 aA | 19.3 ± 6.3 aA | 18.4 ± 1.9 aA |
| SWD | 3.6 ± 0.5 bB | 3.9 ± 0.7 bB | 2.8 ± 0.4 bB | 9.1 ± 2.4 aB | 24.1 ± 3.3 aA | |
| Icatu | WW | 25.6 ± 1.8 aB | 21.1 ± 0.8 aB | 26.7 ± 1.9 aB | 17.4 ± 1.8 aB | 40.1 ± 3.5 aA |
| SWD | 6.2 ± 0.5 bB | 4.1 ± 0.6 bB | 7.9 ± 1.5 bB | 7.6 ± 0.6 bB | 33.8 ± 2.9 aA | |
| Glucose (mg g-1 DW) | ||||||
| CL 153 | WW | 0.63 ± 0.11 aA | 0.93 ± 0.36 bA | 0.42 ± 0.16 aA | 0.49 ± 0.1 aA | 1.36 ± 0.34 aA |
| SWD | 1.81 ± 0.2 aA | 2.32 ± 0.27 aA | 1.44 ± 0.37 aAB | 0.6 ± 0.1 aBC | 0.41 ± 0.07 aC | |
| Icatu | WW | 6.79 ± 1.19 aA | 5.82 ± 2.94 aAB | 2.1 ± 0.86 bB | 1.77 ± 0.17 aB | 2.19 ± 0.21 aB |
| SWD | 7.21 ± 0.85 aA | 4.16 ± 0.55 aAB | 7.36 ± 1.24 aA | 2.12 ± 0.57 aB | 3.56 ± 0.51 aB | |
| Fructose (mg g-1 DW) | ||||||
| CL 153 | WW | 8.43 ± 1.39 aB | 8.97 ± 0.58 aB | 10.75 ± 0.18 aAB | 10.83 ± 0.63 aAB | 13.78 ± 0.59 aA |
| SWD | 9.94 ± 0.85 aA | 7.84 ± 0.68 aA | 8.08 ± 0.38 aA | 9.15 ± 0.4 aA | 7.87 ± 0.93 bA | |
| Icatu | WW | 5.06 ± 0.25 bA | 4.46 ± 0.11 aA | 4.46 ± 0.12 aA | 4.65 ± 0.21 aA | 6.59 ± 0.43 aA |
| SWD | 8.26 ± 0.43 aA | 5.37 ± 0.61 aB | 5.8 ± 0.49 aB | 6.67 ± 0.6 aA | 6.62 ± 0.35 aA | |
| Arabinose (mg g-1 DW) | ||||||
| CL 153 | WW | 1.41 ± 0.21 aC | 2.16 ± 0.2 aBC | 3.29 ± 0.3 aABC | 3.78 ± 0.51 aAB | 4.25 ± 0.44 aA |
| SWD | 2.59 ± 0.42 aA | 2.74 ± 0.3 aA | 2.28 ± 0.29 aA | 1.84 ± 0.3 aA | 1.76 ± 0.61 bA | |
| Icatu | WW | 1.38 ± 0.26 aA | 0.95 ± 0.24 bA | 1.37 ± 0.13 bA | 1.26 ± 0.19 bA | 1.46 ± 0.11 bA |
| SWD | 2.16 ± 0.33 aC | 6.7 ± 0.66 aB | 7.87 ± 0.68 aAB | 9.7 ± 0.6 aA | 8.65 ± 0.66 aA | |
| Mannitol (g g-1) | ||||||
| CL 153 | WW | 13 ± 3.6 bB | 3.7 ± 1 bB | 3.9 ± 0.8 bB | 61.1 ± 22.4 aA | 24.5 ± 2.8 aB |
| SWD | 94.2 ± 12.8 aA | 72.1 ± 8.5 aAB | 90.7 ± 7.2 aA | 45.3 ± 9.4 aBC | 18.7 ± 3.3 aC | |
| Icatu | WW | 8.7 ± 1.2 bA | 5.3 ± 0.6 bA | 3.2 ± 0.9 bA | 5.7 ± 0.6 bA | 5.4 ± 0.1 aA |
| SWD | 109.7 ± 6.3 aA | 98.4 ± 12.9 aA | 65.9 ± 3.8 aB | 98.1 ± 2.4 aA | 10.3 ± 1.2 aC | |
| Total Soluble (mg g-1 DW) | ||||||
| CL 153 | WW | 46.1 ± 5.6 bB | 43.4 ± 2.2 bB | 38.1 ± 4.7 bB | 94.4 ± 18.3 aA | 64.5 ± 4.4 aAB |
| SWD | 112.7 ± 12 aA | 89.6 ± 8.9 aAB | 105.4 ± 6.9 aA | 66.3 ± 7.5 aBC | 51.1 ± 5.8 aC | |
| Icatu | WW | 48.7 ± 2.6 bA | 38.4 ± 2.2 bA | 40.6 ± 3.2 bA | 35.4 ± 2.4 bA | 63.1 ± 4.8 aA |
| SWD | 134.6 ± 5.9 aA | 120.1 ± 12.3 aA | 96 ± 4 aB | 124.5 ± 3.3 aA | 64.5 ± 1.8 aC | |
| Starch (mg glucose equivalents g-1 DW) | ||||||
| CL 153 | WW | 32.5 ± 1.2 aBC | 39.4 ± 2.7 aA | 39 ± 2.1 aAB | 36.8 ± 2.6 aAB | 28.9 ± 1.4 aC |
| SWD | 29 ± 0.3 aAB | 28.4 ± 1.1 bAB | 32.8 ± 0.8 aA | 27.6 ± 0.8 bAB | 25.9 ± 0.5 aB | |
| Icatu | WW | 55.2 ± 1.3 aA | 37.5 ± 3.7 aBC | 44.3 ± 2 aB | 41.5 ± 2.6 aBC | 35.9 ± 1 aC |
| SWD | 39.4 ± 1.3 bA | 36.9 ± 0.5 aA | 35.4 ± 0.4 bA | 24.6 ± 0.5 bB | 34 ± 0.5 aA | |
| Total Soluble/Starch (g g-1) * | ||||||
| CL 153 | WW | 1.42 | 1.1 | 0.98 | 2.56 | 2.23 |
| SWD | 3.89 | 3.15 | 3.21 | 2.4 | 1.97 | |
| Icatu | WW | 0.88 | 1.02 | 0.92 | 0.85 | 1.76 |
| SWD | 3.42 | 3.25 | 2.71 | 5.06 | 1.9 | |
| Total carbohydrates (mg g-1 DW) | ||||||
| CL 153 | WW | 78.6 ± 4.1 bB | 82.8 ± 1.3 aB | 77.1 ± 1.6 bB | 124 ± 11.8 aA | 93.4 ± 5.4 aAB |
| SWD | 141.8 ± 18.1 aA | 118 ± 7.2 aAB | 140.5 ± 6.9 aA | 93.9 ± 10.4 aBC | 77.5 ± 7.6 aC | |
| Icatu | WW | 103.9 ± 1.6 bA | 75.9 ± 2.6 bC | 84.9 ± 0.8 bBC | 77 ± 1.6 bC | 99 ± 3.2 aAB |
| SWD | 173.2 ± 4.6 aA | 159.5 ± 11.9 aAB | 129.5 ± 4.4 aBC | 148.8 ± 3.6 aB | 97.4 ± 2.2 aC | |
* Calculated using the mean values of total soluble sugars and starch.
Conilon Clone 153 (CL153) and Coffea arabica cv. Icatu plants submitted to well-watered (WW) and severe water deficit (SWD), followed by a temperature increase from (25/20°C, day/night), to 42/30°C, and a recovery period of 14 days (Rec14). For each sugar, different letters after the mean values ± SE (n=6) express significant differences among temperature treatments for the same water level (A, B, C), or among water availability levels for each temperature treatment (a, b), always separately for each cultivar.