Figure 2.

Pattern of integration in all bdp1 mutants. Residues between 139 and 241 of Bdp1p are responsible for the altered pattern of Ty1 insertion. Strains expressing each bdp1 allele were transformed with pVIT41, and two independent transformants were induced for transposition. Genomic DNA was prepared from the population of induced cells and insertions were analyzed by PCR with all four primer pairs. (Top two panels) tGly^GCC-specific primer with orientation 1 primer (panel 1) and orientation 2 primer (panel 2). (Bottom two panels) tThr^AGU-specific PCR with orientation 1 primer (panel 3) and orientation 2 primer (panel 4). PCR failed in lane 13 of panel 4. Residues present in the Bdp1p forms are noted at the top. (Lanes 7-10) A bracket denotes the lanes of PCR products from the N-terminal bdp1 deletion strains that show defects in periodic integration.