FIG. 1.

Structure and sequence of the EBD gene. (A) Restriction map of the bovine EBD gene including 0.9 kb of 5′ flanking sequence. E, EcoRI; H, HindIII; P, PstI; UTR, untranslated region; SIG, signal. Also shown is a diagram of the predicted precursor structure of EBD deduced from the gene and cDNA sequences. (B) Nucleotide sequence of the EBD gene and alignment with the TAP gene sequence (13), with carets representing nucleotide identity. Exons (capital letters) were determined by comparison with EBD cDNA sequences (Fig. 2). Consensus sequences for TATA boxes (underlined), NF–IL-6 sites (boldface underlined), and H-APF-1 (double underlined) are indicated (see text). The consensus splice junction residues are shown in boldface. The polyadenylation signal is boxed.