FIG. 6.

Northern blot analysis of several low-abundance β-defensin cDNAs cloned from small intestine by anchored PCR. Total RNAs (20 μg) extracted from 10 different tissues were resolved by denaturing gel electrophoresis, capillary blotted to a nylon filter, and probed with either JR335.B1 as a BNBD-9 probe, BNBD2/3-189a as an BNBD-3 probe, JR300.C7 as a BBD-C7 probe, EBD 285a as an EBD probe, or an α-tubulin probe. The hybridization and wash conditions were as for Fig. 4.