Figure 3.

The inhibition of transcription with THZ1 induces the production of DoGs in pancreatic cancer cells. (A) Browser image of RNA-seq from pancreatic cancer cells treated with DMSO or THZ1, showing the DUSP5 gene that also undergoes the generation of a DoG in response to THZ1. DoG region is indicated by a gray shadow (log₂FC = 3.83 and p-value = 3.06 × 10⁻²⁹); (B) Number of genes that generate DoGs in response to DMSO and THZ1 in pancreatic cancer cells; (C) Venn diagram showing the genes that are shared between pancreatic cancer cells treated with TPL and THZ1 and with breast cancer cells treated with TPL; (D) Heatmap showing the log₂FC of downstream readthrough transcripts (p-value ≤ 0.05) between pancreatic cancer cells treated with THZ1 and DMSO; (E) Scatter plot displaying the log₂FC for the transcript levels of the DoG-producing genes (p-value ≤ 0.05) on the x-axis and the log₂FC for the corresponding DoGs (with p-value ≤ 0.05) on the y-axis. DoG-producing genes were classified as ‘Upregulated’ (38.0%; purple dots) with log₂FC ≥ 1.2, ‘Downregulated’ (27.3%; blue dots) with log₂FC ≤ −1.2, and ‘No change’ (34.8%; gray dots) with log₂FC between 1.2 and −1.2. Percentages for each category are displayed in the legend box. Pearson’s correlation coefficient and p-value were calculated, and both are shown below the graph.