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. Author manuscript; available in PMC: 2024 Jan 22.
Published in final edited form as: Cell Rep. 2023 Dec 13;42(12):113553. doi: 10.1016/j.celrep.2023.113553

Figure 2. Design of hyperglycosylated trihead antigens from additional H1 HAs.

Figure 2.

(A) Diagram of RBD trimer interfaces for TH-SC18, TH-PR34, TH-NC99, and TH-MI15, where mutated residues are colored and labeled.

(B) BLI of trihead components against RBS-directed mAbs (5J8, anti-PR34, and C05) and FluA-20.

(C) Schematic of TH-SC18, TH-PR34, TH-NC99, and TH-MI15 constructs and their in vitro assembly into mosaic or cocktail I53_dn5 nanoparticles.

(D) nsEM 2D class averages of MH-PR34-I53_dn5 and trihead I53_dn5 nanoparticles. Scale bars = 25 nm.

(E) Model structures and gene diagrams for hyperglycosylated triheads with wild-type glycans in light purple and glycan knockins in dark purple. Strain-specific H1 HA numbering is in respective HA strain color, and trihead model numbering is in black.

(F) Reducing SDS-PAGE of wild-type and hyperglycosylated monoheads and triheads without and with PNGaseF digestion.