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. Author manuscript; available in PMC: 2024 Jan 22.
Published in final edited form as: Cell Rep. 2023 Dec 13;42(12):113553. doi: 10.1016/j.celrep.2023.113553

Figure 3. Design and characterization of hypervariable trihead immunogens.

Figure 3.

(A) Sequence conservation among 643 unique H1 sequences (top) and positions mutated in hypervariable library as dark pink (bottom) modeled on the NC99 HA structure (PDB: 7SCN).

(B) TH-NC99–9gly wild-type and hypervariable variants modeled onto the NC99 HA structure (PDB: 7SCN), with all positions mutated in the library shown as sticks, wild-type residues in blue, and mutated residues in magenta.

(C) BLI of triheads and hyperglycosylated triheads, with colored squares around these constructs, and trihead RBS variant components against RBS-directed mAbs (5J8, anti-PR34, and C05) and FluA-20.

(D) Schematic of hypervariable trihead components and assembly into an I53_dn5 nanoparticle.