KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| C05 | Produced in house (Ekiert et al.)6 | N/A |
| 5J8 | Produced in house (Krause et al.)9 | N/A |
| anti-PR34 | Sino Biological | Cat# 11684-R016; RRID:AB_2860374 |
| FluA-20 | Produced in house (Bangaru et al.)21 | N/A |
| Ab6649 | Produced in house (Raymond et al.)36 | N/A |
| Horse anti-mouse IgG, HRP | Cell Signaling Technology | Cat# 7076S; RRID:AB_330924 |
| Goat anti-rabbit IgG, HRP | Cell Signaling Technology | Cat# 7074S; RRID:AB_2099233 |
| Goat anti-human IgG-HRP | Southern Biotech | Cat# 2040–05; RRID:AB_2795644 |
| Bacterial and virus strains | ||
| Influenza reporter viruses | Produced in house (Creanga et al.)80 | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| AddaVax adjuvant | InvivoGen | Cat# vac-adx-10 |
| TMB | SeraCare | Cat# 5120-0083 |
| Papain | Thermo Fisher | Cat# 20341 |
| rProtein A Sepharose fast flow resin | Cytiva | Cat# 17127901 |
| Polyethylenimine (PEI) HCl MAX, Linear, Mw 40,000 | Polysciences | Cat# 24765-1 |
| PNGase F | NEB | Cat# P0705 |
| RDE II | VWR | Cat# 10753-482 |
| Deposited data | ||
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40787 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40788 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40792 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40793 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40794 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40795 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40798 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40800 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40801 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40802 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40831 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40832 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40833 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40834 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40835 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40836 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40837 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40838 |
| Negative-stain EM map of week 6 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_dn5 in complex with MI15 HA-foldon. | EMDataBank | EMD: 40839 |
| Negative-stain EM map of week 22 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with Malaysia54 HA-foldon. | EMDataBank | EMD-40840 |
| Negative-stain EM map of week 22 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with Malaysia54 HA-foldon. | EMDataBank | EMD-40841 |
| Negative-stain EM map of week 22 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with Malaysia54 HA-foldon. | EMDataBank | EMD-40842 |
| Negative-stain EM map of week 22 polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with Malaysia54 HA-foldon. | EMDataBank | EMD-40843 |
| Negative-stain EM map of week 22 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_ dn5 in complex with Malaysia54 HA-foldon. | EMDataBank | EMD-40844 |
| Negative-stain EM map of week 22 polyclonal serum from rabbit immunized with Trihead-Hypervariable-Mosaic-I53_ dn5 in complex with Malaysia54 HA-foldon. | EMDataBank | EMD-40845 |
| Experimental models: Cell lines | ||
| Expi293F | ThermoFisher | Cat# A14527 |
| MDCK-SIAT-PB1 | Produced in house (Creanga et al.)80 | N/A |
| Experimental models: Organisms/strains | ||
| BALB/c mice | Jackson Laboratory | Cat# 000651 |
| NZW rabbits | Labcorp, Inc. | https://www.labcorp.com/ |
| Recombinant DNA | ||
| See Table S1 for amino acid sequences | ||
| Software and algorithms | ||
| Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| EPU | Thermo Fisher | https://www.thermofisher.com/us/en/home/electron-microscopy/products/software-em-3d-vis/epu-software.html |
| CryoSparc | (Punjani et al.)76 | https://cryosparc.com/ |
| UCSF Chimera | (Pettersen et al.)81 | https://www.cgl.ucsf.edu/chimera/ |
| UCSF ChimeraX | (Goddard et al.)82 | https://www.rbvi.ucsf.edu/chimerax/ |
| PyMol | Schrodinger, LLC | https://pymol.org/2/ |
| RosettaScripts “sugarcoat” code | (Adolf-Bryfogle et al.)34 | N/A |
| Unicorn 7.0 | GE Healthcare | http://www.gelifesciences.com/ |
| Other | ||
| 300 mesh grids | Ted Pella | Cat# 01843-F |
| Filter paper | Cytiva | Cat# 1004047 |
| Uranyl formate | SPI Chem | Cat# 02545-AA |
| Superdex 200 Increase SEC column | Cytiva | Cat# 28-9909-44 |
| Superose 6 Increase SEC column | Cytiva | Cat# 29091596 |
| Talon resin | TaKaRa | Cat# 635652 |
| Excel resin | Cytiva | Cat# 17371203 |
| Isoflurane | USP Patterson | Cat# 07-893-1389 |
| EndoSafe LAL Test Cartridges | Charles River Labs | Cat# PTS20005F |
| Lemo21(DE3) | New England BioLabs | Cat# C2528J |
| MAX Efficiency™ DH5α Competent Cells | Sigma-Aldrich | Cat# I6758 |
| Isopropyl-B-D-thiogalactoside (IPTG) | Thermo Fisher | Cat# 18258012 |
| Kanamycin Sulfate | Sigma-Aldrich | Cat# K1876 |
| HisTrap HP | Cytiva | Cat#17-5247-01 |
| Octet Protein A Biosensors | Sartorius | Cat# 18-5010 |
| Turkey Red Blood Cells | Lampire | Cat# 7249409 |
| Plasmid Plus Maxi Kit | Qiagen | Cat# 12963 |
| Trypsin, TPCK Treated | Thermo Fisher | Cat# 20233 |