Figure 2. Design and characterization of trihead nanoparticles with varied antigen spacing.
(A) Models of trihead nanoparticles with various extension domains, monohead-I53_dn5, and HA-I53_dn5. The models are colored blue, gray, and orange, respectively, for HA-derived segments, I53_dn5B/GCN4, and I53_dn5A.
(B) BLI of trihead nanoparticle extension series components against C05, FluA-20, and Ab6649.
(C) DLS of trihead nanoparticle extension series.
(D) nsEM micrographs of trihead nanoparticle extension series. Scale bars: 50 nm.